![]() The prepared sensor was incubated in 40 μL of TSH for 30 min at room temperature and washed with PBS. The unreacted active surface groups were blocked by incubating the functionalized electrode with 0.1% (w/w) bovine serum albumin (BSA) for 1 h at room temperature, followed by washing with PBS. Unbound antibodies were washed away using PBS. The modified electrode was then rinsed with phosphate buffer 10 mM pH 7.4 and incubated in a solution of anti-TSH overnight to yield sensing interfaces. After rinsed with Milli-Q water to remove physically adsorbed MPA, it was incubated in a solution with 20 mg/mL of EDC and 10 m/mL of NHS for 1 h at room temperature. The cleaned electrode was incubated overnight at room temperature in a 2 mM solution of MPA aqueous solution. Ag/AgCl) once, at 50 mV/s scan rate, and then rinsed in Milli-Q water. The electrode potential was swept from −200 to −1200 mV (vs.
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